Regulatory

Part:BBa_K3753011:Design

Designed by: Yiqing Zhang   Group: iGEM21_NJTech_China   (2021-10-16)
Revision as of 14:07, 16 October 2021 by RI2020 (Talk | contribs) (→‎Source)


UASCLB-pTEF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 747
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 747
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 747
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 747
    Illegal NgoMIV site found at 1269
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

UASCLB-pTEF is synthesized by fusing three UASCLB tandem to the tef2 promoter (BBa_K3753003). UASCLB was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The amplified product is a 240bp sequence upstream of the clb2 promoter start codon.

Source

Saccharomyces cerevisiae

References