Coding

Part:BBa_K3853013

Designed by: RuYi Ma   Group: iGEM21_CPU_CHINA   (2021-09-20)
Revision as of 22:18, 20 October 2021 by Lp-tiffany (Talk | contribs)


MnP (E74P)

Manganese peroxidase (MnP) is the key enzyme in our degrading system. In order to improve its catalyzing ability, we tried rational design. And according to the computational redesign results, 6 mutants were chosen and tested, including their relative enzyme activity and the effect of temperature/pH/organic solvents on them. MnP(E74P) is one of the most promising mutant of MnP. We use BBa_K3853056 to construct the expression system to express and purify the protein.

F o l d   d i f f e r e n c e = 2 C t ( A C T 1 ) − C t ( S p y T a g − M n P )

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None