Part:BBa_K3846021
Diphosphomevalonate decarboxylase (MVD) - MBIS pathway
This part features the codon-optimised coding sequence of diphosphomevalonate decarboxylase (MVD) from Homo sapiens. This enzyme catalyses the ATP dependent decarboxylation of (R)-5-diphosphomevalonate to form isopentenyl diphosphate (IPP) as part of the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoids and sterol synthesis.
The corresponding phytobrick is BBa_K3846123
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
iGEM Hamburg 2021 part collection
Terpenoids are an important group of natural products used as biofuels, drugs or fragrances. Naturally occuring in plants it has been shown that microbial terpene production in microorganisms like yeast, E. coli or cyanobacteria is possible. Nevertheless iGEM projects seem to rarely focus on this interesting class of natural products which is correlated with a lack of useful parts inside the iGEM registry.
Fortunately we were able to change that and designed a novel golden gate based toolbox which allows.
- production of terpenoid precursors and simple terpenoids
- creation of CYP P450-reductase fusion enzymes to optimise processing of terpenoid precursors and production of bioactive target products
- modularity of the system to enable exchange of linker sequences/promoters/etc. (MoClo-compatible toolbox)
MoClo-based Part Design 2.0
To improve the usefulness of our parts, we then aimed to make our parts compatible with the MoClo standard of goten gate based IIS restriction enzyme assembly. Thereby we expanded the Common Genetic Syntax for fusion sites to allow the creation of a) fusion proteins connected by linker sequences and b) multiple CDS expressed in an operon. More useful information and an overview of all our parts can be found on our wiki.
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