Part:BBa_K3788021

Expression of GFP and of the timer device fused to the RFP protein

Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa and cal. caa gene is coding for Colicin A (ColA) a toxin toxin produced by E. coli strains, and active against other non-immune E.coli strains. In this contruction GFP permit to understand the mechanism, and ColA isn't able to have his toxin activity. To protect themselfs, E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis. In this contruction RFP permit to understand the mechanism, and Cal isn't able to have his lysis activity. The regulation is complex and involves many regulatory elements: terminator (+) and (-), promoters, antisense coding sequence, etc.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1528
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1848
  Illegal BamHI site found at 1521
  Illegal XhoI site found at 1397
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1597
  Illegal AgeI site found at 2849
  Illegal AgeI site found at 2961
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 867
  Illegal SapI.rc site found at 1351