Part:BBa_K3788002
6His-P20
BBa_K3788002 sequence is coding for P20 a chaperone protein in Bacillus thuringiensis. This chaperon has a 6x Histine tag in N-ter to allow purification of the protein P20and specific detection by Western blot. The gene p20 was optimised for E. coli K12.
Expected size :
6his_p20 gene is 570bp
6His_P20 protein is 20 kDa
P20 chaperon:
Was discovered in B.thuringiensis, a p20 gene in operon with cry11Aa and <i<cyt1Aa</i>. Thus, the P20 protein was shown to be necessary for the formation of inclusion bodies of Cry11Aa and Cyt1Aa. P20 would allow a greater production of these toxins and allow their stability (Cyt1Aa would be protected from the proteolytic action before its maturation). The co-production of P20 and Cyt1Aa in E.coli would limit its toxic effects in the producer cell.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 154
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |