Coding

Part:BBa_K3788002

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)
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6His-P20


BBa_K3788002 sequence is coding for P20 a chaperone protein in Bacillus thuringiensis. This chaperon has a 6x Histine tag in N-ter to allow purification of the protein P20and specific detection by Western blot. The gene p20 was optimised for E. coli K12.

Expected size :

6his_p20 gene is 570bp

6His_P20 protein is 20 kDa


P20 chaperon:

Was discovered in B.thuringiensis, a p20 gene in operon with cry11Aa and <i<cyt1Aa</i>. Thus, the P20 protein was shown to be necessary for the formation of inclusion bodies of Cry11Aa and Cyt1Aa. P20 would allow a greater production of these toxins and allow their stability (Cyt1Aa would be protected from the proteolytic action before its maturation). The co-production of P20 and Cyt1Aa in E.coli would limit its toxic effects in the producer cell.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 154
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None