Coding

Part:BBa_K3788023:Design

Designed by: Rebecca Pags   Group: iGEM21_Aix-Marseille   (2021-09-28)
Revision as of 00:06, 22 October 2021 by Rpages (Talk | contribs)

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Expression of GFP and of an optimized design of the timer device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1568
    Illegal PstI site found at 1671
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2266
    Illegal PstI site found at 1568
    Illegal PstI site found at 1671
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2586
    Illegal BamHI site found at 2259
    Illegal XhoI site found at 2135
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1568
    Illegal PstI site found at 1671
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1568
    Illegal PstI site found at 1671
    Illegal NgoMIV site found at 2335
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 867
    Illegal SapI.rc site found at 2089


Design Notes

Source

References

- Cascales, E. et al. Colicin biology. Microbiol. Mol. Biol. Rev. MMBR71, 158–229 (2007).

- Cavard, D. Role of Cal, the colicin A lysis protein, in two steps of colicin A release and in the interaction with colicin A-porin complexes. Microbiol. Read. Engl. 150, 3867–3875 (2004).

- Lloubes, R., Baly, D. & Lazdunski, C. The promoters of the genes for colicin production, release and immunity in the ColA plasmid: effects of convergent transcription and Lex A protein. Nucleic Acids Res . 14, 2621–2636 (1986).

<a class=”bg-transparent” href=”https://www.prodoric.de/vfp.”>VirtualFootprint - PRODORIC</a>