Coding

Part:BBa_K3788002:Experience

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)
Revision as of 16:57, 15 October 2021 by Rpages (Talk | contribs)


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Applications of BBa_K3788002

PCR amplification of Ghisp20 gene :

The DNA sequence of Ghisp20 was amplify by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is 570bp and a +/- 600bp band is observed. Amplification of DNAs sequences with primers were validated.

T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png

Figure. Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning. a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 bp), flagcry11Aa (expected 1847 bp), strepcyt1Aa (expected 775 bp) and 6hisp20 (expected 568 bp); L (SMART Ladder, its size is shown on the right side).


User Reviews

UNIQ4ee1df8ecfce794a-partinfo-00000000-QINU UNIQ4ee1df8ecfce794a-partinfo-00000001-QINU