Part:BBa_K3984001
laccase
Degrading antibiotics
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 73
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 782
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1230
Illegal NgoMIV site found at 1596
Illegal AgeI site found at 193 - 1000COMPATIBLE WITH RFC[1000]
Profile
Origin: Pleurotus ostreatus（P. ostreatus HAUCC 162）
Properties: Degradation of sulfadiazine
Usage and Biology
Laccase is an oxidoreductase that uses oxygen as an electron acceptor. It can oxidize a variety of aromatic compounds and produce water as a by-product. In the 19th century, humans first discovered laccase in Japanese sumac. Because of its outstanding application value in biotechnology applications, scientists have gradually deepened their research on laccase. They discovered that laccase is also present in animals and microorganisms. Laccases derived from different hosts have different functions in the body. Among them, fungal laccases derived from white rot fungi, Trametes, Pleurotus ostreatus and other fungal laccases occupy the mainstream of the current laccase application process. This type of laccase has a relatively wide range of substrate specificity during the application process, and does not require hydrogen peroxide. With the participation of, a variety of phenolic compounds and their derivatives can be directly oxidized with oxygen as the electron acceptor.
In recent years, the main methods for removing antibiotic residues from different sources are traditional methods such as high-temperature composting, fermentation; oxidation, adsorption, electrochemical treatment, membrane method, and microbial degradation. However, these methods have the disadvantages of low removal efficiency, high cost of use, and the possibility of secondary pollution to the environment. Moreover, these methods cannot fundamentally solve the problem of antibiotic residues in poultry. Therefore, it is necessary to find a direct, effective and low-cost method to remove antibiotic residues in poultry. At present, many studies have shown that fungal laccase plays an important role in the process of treating antibiotic residues in wastewater. Three laccase-producing genes LACC6, LACC9, and LACC10 obtained from Pleurotus ostreatus were heterologously expressed in Pichia pastoris and found that the efficiency of degrading sulfa antibiotics reached more than 97%.
| None |