Coding

Part:BBa_K3927017

Designed by: Chew Chin Wei   Group: iGEM21_NUS_Singapore   (2021-10-13)
Revision as of 12:41, 14 October 2021 by CWChew (Talk | contribs) (→‎Characterization)


Human Beta Defensin 2 (S. cerevisiae)

Coding sequence for the human immune protein, human beta defensin 2. Codon optimized for S. cerevisiae).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

This part contains the CDS for Human Beta Defensin 2 (HBD2) with a 6xHis-tag attached to the C-terminus of the peptide sequence.

Usage

This part contains only the CDS and 6xHis-tag. Stop codon is required at the end of the sequence as it is not included.

Promoter and terminator is required for successful expression, additional secretion signal peptide may be required to successfully express and purify HBD2 from culture.

Design

Characterization

Part characterization was done using the composite part BBa_K3927018 (HB2 with mating factor alpha secretion tag). Please refer to that part page for more details.


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Categories
Parameters
None