Project

Part:BBa_K3982030:Design

Designed by: Akankshya Ramkrishna Sahu   Group: iGEM21_IISER_Berhampur   (2021-10-01)
Revision as of 18:31, 14 October 2021 by Akankshya16 (Talk | contribs)


CODE M Construct C2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 124
    Illegal XbaI site found at 148
    Illegal PstI site found at 160
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 124
    Illegal PstI site found at 160
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 124
    Illegal BglII site found at 598
    Illegal BamHI site found at 142
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 124
    Illegal XbaI site found at 148
    Illegal PstI site found at 160
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 124
    Illegal XbaI site found at 148
    Illegal PstI site found at 160
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

sgRNA sequence BBa_K3982004 inserted using SDM

Source

Addgene #15030

References