Regulatory

Part:BBa_K3927003

Designed by: Chew Chin Wei   Group: iGEM21_NUS_Singapore   (2021-10-13)
Revision as of 03:18, 18 October 2021 by Hung Truong (Talk | contribs) (→‎Description)


EL222 Expression Driver

This composite part encodes for a 3C120-CYC-LacO promoter (BBa K3927002 ) controlling EL222 for a feed forward circuit, with Part BBa_K2407003 as a terminator

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 670
    Illegal XbaI site found at 203
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 670
    Illegal NheI site found at 185
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 670
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 670
    Illegal XbaI site found at 203
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 670
    Illegal XbaI site found at 203
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

The EL222 expression driver is a composite part consisting of parts BBa_K3927002(3C120-CYC-LacO), BBa_K3570021(NLS-VP16-EL222) and BBa_K2407003(CYCt). 3C120-CYC-LacO is activated by dimerization of NLS-VP16-EL222 in the presence of blue light, which further increases the amount of NLS-VP16-EL222 produced by this part. The amount of NLS-VP16-EL222 then increases in a positive feedback loop, activating both the 3C120-CYC-LacO within this part as well as any other parallel, C120 motif-containing promoters until blue light is removed and NLS-VP16-EL222 un-dimerizes.

Usage

Design

Characterization

[edit]
Categories
Parameters
None