DNA

Part:BBa_K313010

Designed by: Ryosuke Kamei, Ryo Kariyazono, Takashi Matsumoto, Takashi Hiroi, Mei Kobayashi, Yuka Yashiro, Tomoka Ao   Group: iGEM10_UT-Tokyo   (2010-10-22)
Revision as of 09:50, 13 October 2021 by Teapot657 (Talk | contribs)

loading sequence of RNA phage MS2

This sequence is necessary for loading of RNA into the capsid of RNA phage MS2. The coat proteins of phage MS2 is known to aggregate around this sequence.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_MS2 Phage MS2] assay page.

We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.


We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.

Applications of BBa_K313010

We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.

2D stucture

UNIQ1d5331b5ff629d99-partinfo-00000001-QINU UNIQ1d5331b5ff629d99-partinfo-00000002-QINU
We use RNAfold and StructureEditor to predict 2D structure of our circRNA, which contains MS2 aptamer.The results are below:

MS2 aptamer on the circRNA scaffold.png
Figure 1.2D stucture predicted by RNAfold

SYSU-CHINA circRNA-1.png
Figure 2.2D stucture predicted by StructureEditor

3D stucture

UNIQ1d5331b5ff629d99-partinfo-00000003-QINU UNIQ1d5331b5ff629d99-partinfo-00000004-QINU
We use this website:http://biophy.hust.edu.cn/new/3dRNA/create, to predict the 3D structure of our circRNA, which contains MS2 aptamer.The result lists below:


SYSU-CHINA circRNA-2.png

Figure 3. 3D stucture of MS2 aptamer predicted by Xiaolab

MS2 binding mechanism

UNIQ1d5331b5ff629d99-partinfo-00000005-QINU UNIQ1d5331b5ff629d99-partinfo-00000006-QINU


MS2 binding mechanism.png

Figure 4. MS2 binding mechanism


split EGFP

UNIQ1d5331b5ff629d99-partinfo-00000007-QINU UNIQ1d5331b5ff629d99-partinfo-00000008-QINU
We are SYSU-CHINA,and this year we use this part as MS2 coat protein's aptamer to do a split EGFP experiment,for the proof of concept.Here we found a research article, which use liner RNA with MS2 and PP7 aptamer, to do the split EGFP experiment.Below are their experimental data.


The principle of spite EGFP.png

Figure 3.the principle and experimental data of split EGFP


UNIQ1d5331b5ff629d99-partinfo-00000009-QINU
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