DNA
Part:BBa_K3822003
Designed by: Jiajia Li Group: iGEM21_YiYe-China (2021-09-17)
Hypermethylated region of TFPI2
The sequence of Hypermethylated region of TFPI2.We use this part for detection of colorectal cancer (CRC).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 6
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
In our project, the trigger sequence is linked to this part by DNA amplification and was added into the cell free system together with the plasmid contain toehold sequence, the report fluorescent protein will be expressed. The visible color and fluorescent signal can indicate the methylation degree of the TFPI2 gene in CRC patients.
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Categories
Parameters
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