Coding

Part:BBa_K3853008

Designed by: Peng Luo   Group: iGEM21_CPU_CHINA   (2021-09-19)
Revision as of 20:00, 12 October 2021 by Lp-tiffany (Talk | contribs)


his-tag-SpyTag-MnP

Manganese Peroxidase (MnP) is a highly glycosylated lignin peroxidase with heme. This is the version with SpyTag. In order to be able to construct our multi-enzyme complex, we introduced the SpyTag/SpyCatcher system. We added SpyTag to the N-terminus of each protein throught 10 × ELP, so it can be combined with dCas9-SpyCatcher through covalent isopeptide action, thereby being immobilized On dsDNA. His-tag was added to purify the protein. We use BBa_K3853051 to construct the expression system and to express and to purify the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part
Parameters
None