Tag

Part:BBa_K3782002

Designed by: Jana Nf   Group: iGEM21_UNILausanne   (2021-10-11)
Revision as of 15:08, 11 October 2021 by Jana1907 (Talk | contribs)

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6XHis-Tag

6xHis-Tag for Purification and Identification of Protein of Interest

The His-Tag is a commonly used sequence both as a reporter and as a purification tag. Its sequence, consisting of 6 histidine residues, has a very high affinity to nickel(II) ions and is therefore used in affinity purification columns with immobilized nickel ions. The binding of the His-Tag to the nickel ions is not efficient at acidic pH values, and any purification process should therefore be done above pH=7. Imidazole is used to elute the protein from the column by competing with the His tag on binding to the immobilized Nickel ions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None