Reporter

Part:BBa_K3962340:Design

Designed by: Siheng Li   Group: iGEM21_Leiden   (2021-10-11)
Revision as of 14:19, 15 October 2021 by SihengLi (Talk | contribs)

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pBAD promoter with mCherry for inducible expression of reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

We designed this construct to calibrate the expression of pBAD to a standardized constitutive promoter. Codon optimization was performed for the expression of the gene in E. coli.


Source

All subparts are from iGEM registry.

References