DNA
Part:BBa_K3791012:Design
Designed by: Laura Sánchez Ruiz, Auba Fuster Palŕ Group: iGEM21_UPF_Barcelona (2021-10-10)
Revision as of 11:57, 10 October 2021 by Laurasanchezruiz (Talk | contribs)
gRNA Erythromycin construct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The repeat sequence must be placed before the spacer sequence, that is, in the 5â extreme.
Source
The repeat and the T7 promoter sequences were extracted from the parts registry (BBa_K2927006) (BBa_K1614000), whereas the spacer one was obtained from being the complementary sequence of an erythromycin-resistant gene DNA fragment. (as explained in its own part page: BBa_K3791002).
References
[1] New England Biolabs. (s/f). How do I design a guide RNA for use with EnGen Lba Cas12a? Neb.com. From: https://international.neb.com/faqs/2018/05/03/how-do-i-design-a-guide-rna-for-use-with-engen-lba-cas12a