![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3885202
Designed by: Xue Li Group: iGEM21_ZJUT-China (2021-10-10)
P70-σ28
Under the influence of the P70 promoter, the transcription factor σ28 is produced and activates expression from the P28 promoter
Usage and Biology
p70-σ28 is a significant part of genecircuit where p70 can be regulated by the σ70 factor present in cell-free system. The rest of the parts in the gene circuit are dependent on the expression of the σ28 factor to initiate expression. Recognition sequence from Escherichia coli str. K-12 substr.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 487
Illegal SapI.rc site found at 512
Illegal SapI.rc site found at 668
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Categories
Parameters
None |