RNA

Part:BBa_K3885171:Design

Designed by: Xue Li   Group: iGEM21_ZJUT-China   (2021-10-10)
Revision as of 02:58, 13 October 2021 by Snow9 (Talk | contribs) (→‎References)

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Interacting Guide RNA（igRNA）


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our project, we add a special sequence in the 5' end of the gRNA, which consists of recognition sequence A, cis-repressed sequence and recognition sequence B. The cis-repressed sequence is complementary to the spacer, leading to the repression of the binding ability between igRNA with its DNA target. The recognition sequence A and the recognition sequence B are complementary to the interacting sequence A and interacting sequence, respectively.


Source

none

References

Galizi R, Duncan JN, Rostain W, Quinn CM, Storch M, Kushwaha M, Jaramillo A. Engineered RNA-Interacting CRISPR Guide RNAs for Genetic Sensing and Diagnostics. CRISPR J. 2020 Oct;3(5):398-408.