Composite

Part:BBa_K3755029:Design

Designed by: Qi Xin   Group: iGEM21_ShanghaiTech_China   (2021-10-01)
Revision as of 09:08, 9 October 2021 by HeteroauXin (Talk | contribs) (→‎References)

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cfa-c-mfp5 CDS-his tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 355
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a complete CDS for 3*repeat Mfp5 protein expression and it use be uesd with part:BBa_K3755030. However, the start codon and stop codon is not contained in the sequence. So please add the start codon and the stop codon to the beginning and the end of the sequence before you put this part into the protein expression plasmid(which we recommend pET28).

Source

From literature of Eugene Kim's team from Washington University in St. Louis.

References

1]Carvajal-Vallejos P, Pallissé R, Mootz HD, Schmidt SR. Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources. J Biol Chem. 2012 Aug 17;287(34):28686-96. doi: 10.1074/jbc.M112.372680. Epub 2012 Jun 28. PMID: 22753413; PMCID: PMC3436554.
[2]Stevens AJ, Brown ZZ, Shah NH, Sekar G, Cowburn D, Muir TW. Design of a Split Intein with Exceptional Protein Splicing Activity. J Am Chem Soc. 2016 Feb 24;138(7):2162-5. doi: 10.1021/jacs.5b13528. Epub 2016 Feb 8. PMID: 26854538; PMCID: PMC4894280.
[3]Kim E, Dai B, Qiao JB, Li W, Fortner JD, Zhang F. Microbially Synthesized Repeats of Mussel Foot Protein Display Enhanced Underwater Adhesion. ACS Appl Mater Interfaces. 2018 Dec 12;10(49):43003-43012. doi: 10.1021/acsami.8b14890. Epub 2018 Nov 27. PMID: 30480422.