RBS

Part:BBa_K3726093:Design

Designed by: Lucia Tapia   Group: iGEM21_MADRID_UCM   (2021-10-08)
Revision as of 15:41, 8 October 2021 by LuciaTapia (Talk | contribs) (→‎References)

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RBS*


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RBS was designed via an analysis of the RBS sequences of all coding genes longer than 100 amino acids in Synechocystis PCC 6803. It was found that only 26% of the genes contained the core SD sequence, while in E. coli it was 57% (Ma et al., 2002). Based on that the consensu RBS sequence TAGTGGAGGT which is complementary to the anti-SD sequence AUCACCUCCUUU of Synechocystis PCC 6803 has been designed.


Source

Coding sequence of this RBS has been found within the genome of Synechocystis PCC 6803.


References

D. Camsund and P. Lindblad, "Engineered Transcriptional Systems for Cyanobacterial Biotechnology", Frontiers in Bioengineering and Biotechnology, vol. 2, 2014.

T. Heidorn, D. Camsund, H. Huang, P. Lindberg, P. Oliveira, K. Stensjö and P. Lindblad, "Synthetic Biology in Cyanobacteria", Methods in Enzymology, pp. 539-579, 2011.