Coding
tal

Part:BBa_K4047034:Design

Designed by: An Nguyen   Group: iGEM21_MiamiU_OH   (2021-10-06)
Revision as of 21:31, 20 October 2021 by Imesam (Talk | contribs)

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transaldolase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 685
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 520
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1144


Design Notes

This coding region was sourced from the Synechococcus elongatus PCC 7942 Genbank genome sequence for transaldolase to maximize efficiency within the same organism as a chassis. This enzyme is natively expressed within the organism and plays a role in its central metabolism, catalyzing the reversible conversion of fructose-6-phosphate and erythrose-4-phosphate to glyceraldehyde-3-phosphate and sedoheptulose-7-phosphate. This reaction is part of the Calvin-Benson-Bassham cycle, making it relevant to future work altering photosynthetic reactions.

This coding region does show homology to other organisms, but is not well characterized outside of Synechoccocus species.

NCBI blast results for the transaldolase BBa_K4047034 part sequence, showing current recognized homology to other organisms.

Source

Sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for transaldolase, at the locus tag Synpcc7942_2297. https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2367528&to=2368721&report=fasta&strand=2



References