Reporter

Part:BBa_K3889000

Designed by: Prameya Garge, Ashwin Sharma   Group: iGEM21_IISER-Tirupati_India   (2021-09-26)
Revision as of 08:19, 6 October 2021 by AshWinShaRma (Talk | contribs)


Azurite_BFP (BFP.B3)

Azurite is a Blue Fluorescence Reporter protein from Aequorea victoria and has the following parameters [1,2]:

Ex : Peak excitation wavelength (in λ) 383
Em: Peak emission wavelength (in λ) 447
EC: Extinction Coefficient (M-1 cm-1) 26,200
QY: Quantum Yield 0.55
Brightness 14.41
pKa 5.0
Maturation (min) 18.0
Lifetime (ns) 3.65



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Mena, M. A., Treynor, T. P., Mayo, S. L., & Daugherty, P. S. (2006). Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library. Nature biotechnology, 24(12), 1569–1571. https://doi.org/10.1038/nbt1264

2. https://www.fpbase.org/protein/azurite/

[edit]
Categories
Parameters
None