Coding

Part:BBa_K3726021:Design

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-05)
Revision as of 14:23, 5 October 2021 by Sbarraga (Talk | contribs)


CDS_PKPa


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2059
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2059
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 598
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2059
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2059
    Illegal NgoMIV site found at 175
    Illegal NgoMIV site found at 2284
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part corresponds with the codon optimized coding sequence of PKPa. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases



Source

Coding sequence of this Xylulose-5-phosphate phosphoketolase has been found within the genome of Pseudomonas aeruginosa. Uniprot reference: https://www.uniprot.org/uniprot/A0A2R4KQ27


References

W. Xiong et al., "Phosphoketolase pathway contributes to carbon metabolism in cyanobacteria", 2021.

I. Bogorad, T. Lin and J. Liao, "Synthetic non-oxidative glycolysis enables complete carbon conservation", 2021.