Coding

Part:BBa_K3726001:Design

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-03)
Revision as of 13:26, 5 October 2021 by Sbarraga (Talk | contribs) (→‎References)


CDS_PhaB^173S


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part corresponds with the codon optimized coding sequence of PhaB^173S enzyme. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942.

Source

Coding sequence of this Acetoacetyl-CoA reductase has been found within the genome of Ralstoni eutropha. Uniprot reference: https://www.uniprot.org/uniprot/P14697

References

"Directed Evolution and Structural Analysis of NADPH-Dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) Reductase from Ralstoni eutropha Reveals Two Mutations Responsible for Enhanced Kinetics | Applied and Environmental Microbiology", Applied and Environmental Microbiology, 2021. [Online]. Available: https://journals.asm.org/doi/10.1128/aem.01768-13?permanently=true.