Composite

Part:BBa_K3814071

Designed by: Simon Tang   Group: iGEM21_Sydney_Australia   (2021-10-01)
Revision as of 00:36, 22 October 2021 by Simontang (Talk | contribs)

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Cluster 3

We aim to produce naturally transformable (NT) E. coli by inserting the NT genes of another species into it. We have chosen 23 genes from Acinetobacter baylyi and have planned to insert them into the fliK gene in the E. coli.

To do this, we have devised a novel recombineering strategy that allows for homologous recombination to insert large amounts of DNA sequentially into the chromosome. Thus, rather than inserting each of the 23 genes individually, we can transform them in clusters of genes.

We determined these clusters by assessing the biological function of each gene and through k-means clustering, and one such is shown here.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 4216
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4268
    Illegal NheI site found at 4291
    Illegal SpeI site found at 4216
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4731
    Illegal XhoI site found at 75
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 4216
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 4216
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 691


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