Part:BBa_K3817015

This part is a cascade of temperature control promoters and toxin-antitoxin systems. With this part, we can achieve both toxin and antitoxin expression at the optimal temperature for E. coli so that it does not commit suicide. However, when the temperature is below 30°C, the promoter is repressed and the antitoxin is not expressed, so the toxin kills the cell and the biosafety module is achieved.