Coding
cas7

Part:BBa_K3776014

Designed by: Helene Heling Huang   Group: iGEM21_HK_SSC   (2021-10-01)
Revision as of 18:07, 17 October 2021 by Meowstarperson (Talk | contribs)

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cas7

This Cas7 protein from Vibrio cholerae is part of the insertion of transposable elements by guide RNA–assisted targeting (INTEGRATE) system devised by Vo et al.(2020). The INTEGRATE system consists of CRISPR RNA-guided integrases for bacterial genome engineering (see BBa_K3776022). In this system, Cas7 is part of the TniQ-Cascade protein complex which is necessary for RNA-guided DNA integration.(Klompe et al., 2019)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 381
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None