Coding

Part:BBa_K3861022:Design

Designed by: Mario Delgadillo   Group: iGEM21_Humboldt_Berlin   (2021-10-01)
Revision as of 18:08, 20 October 2021 by Deguevam (Talk | contribs)


hilA transcriptional regulator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1112
    Illegal PstI site found at 1309
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1112
    Illegal PstI site found at 1309
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1635
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1112
    Illegal PstI site found at 1309
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1112
    Illegal PstI site found at 1309
    Illegal NgoMIV site found at 151
    Illegal AgeI site found at 1258
  • 1000
    COMPATIBLE WITH RFC[1000]

atgccacattttaatcctgttcctgtatcgaataaaaaattcgtctttgatgatttcatactcaacatggacggctccctgctacgctcagaaaagaaagtcaatattccgccaaaagaatatgccgttctggtcatcctgctcgaagccgccggcgagattgtgagtaaaaacaccttactggaccaggtatggggcgacgcggaagttaacgaagaatctcttacccgctgtatttatgccttacgacgtattctgtcggaagataaagagcatcgttacattgaaacactgtacggacagggctatcggtttaatcgtccggtcgtagtggtgtctccgccagcgccgcaacctacgactcatacattggcgatacttccttttcagatgcaggatcaggttcaatccgagagtctgcattactctatcgtgaagggattatcgcagtatgcgccctttggcctgagcgtgctgccggtgaccattacgaagaactgccgcagtgttaaggatattcttgagctcatggatcaattacgccccgattattatatctccgggcagatgatacccgatggtaatgataatattgtacagattgagatagttcgggttaaaggttatcacctgctgcaccaggaaagcattaagttgatagaacaccaacccgcttctctcttgcaaaacaaaattgcgaatcttttgctcagatgtattcccggacttcgctgggacacaaagcagattagcgagctaaattcgattgacagtactatggtttacttacgcggtaagcatgagttaaatcaatacaccccctatagcttacagcaagcgcttaaattgctgactcaatgcgttaacatgtcgccaaacagcattgcgccttactgtgcgctggcagaatgctacctcagcatggcgcaaatggggatttttgataaacaaaacgctatgatcaaagctaaagaacatgcgattaaggcgacagagctggaccacaataatccacaagctttaggattactggggctaattaatacgattcactcagaatacatcgtcgggagtttgctattcaaacaagctaacttactttcgcccatttctgcagatattaaatattattatggctggaatcttttcatggctggtcagttggaggaggccttacaaacgattaacgagtgtttaaaattggacccaacgcgcgcagccgcagggatcactaagctgtggattacctattatcataccggtattgatgatgctatacgtttaggcgatgaattacgctcacaacacctgcaggataatccaatattattaagtatgcaggttatgtttctttcgcttaaaggtaaacatgaactggcacgaaaattaactaaagaaatatccacgcaggaaataacaggacttattgctgttaatcttctttacgctgaatattgtcagaatagtgagcgtgccttaccgacgataagagaatttctggaaagtgaacagcgtatagataataatccgggattattaccgttagtgctggttgcccacggcgaagctattgccgagaaaatgtggaataaatttaaaaacgaagacaatatttggttcaaaagatggaaacaggatccccgcttgattaaattacggtaa


Design Notes

Source

Coding region of hilA in Salmonella Typhimurium ATCC14028.

References