Plasmid

Part:BBa_K3861005

Designed by: Kristin Funke   Group: iGEM21_Humboldt_Berlin   (2021-10-01)
Revision as of 14:58, 17 October 2021 by Martje1998 (Talk | contribs)


pKF01

iGEM Humboldt_Berlin 2021 standard backbone based on pSB1C3. It contains the standard pSB1C3 ORI, the <a herf="https://parts.igem.org/Part:BBa_K3861003">thyA CDS</a> and the <a href="https://parts.igem.org/Part:BBa_K3861004">lldR CDS</a>. We used this backbone as part of the composite part / backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861026">pKF02</a>.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 997
    Illegal XhoI site found at 2804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None