Part:BBa_K3888013

HupS Knockout Uptake hydrogenase is a hydrogenase that recycles hydrogen produced by nitrogenase and converts it back into protons, whose expression reduces hydrogen production. The uptake hydrogenase is composed of small and large subunits, encoded by hupS and hupL, respectively. HupS mediates electron transport from the active site of the large subunit to redox partners and downstream reactions through a set of FeS clusters. HupL harbors the active site that contains the metals Ni and Fe.

The HupS gene encodes a partial uptake hydrogenase subunit. Mutating the hupS gene inactivated the uptake hydrogenase enzyme, thus increasing hydrogen production. The experimental plan is to obtain a 1.3 kb DNA fragment containing hupS gene and about 0.5 kb upstream and downstream flanking region DNA fragment by PCR, and clone them into pJQ200SK plasmid. The engineered plasmid will then be transferred by e. coli S17-1 conjugation or electroporation into R. Palustris CGA009

HupS Knockout

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]