Coding
panS

Part:BBa_K3776007:Design

Designed by: Helene Heling Huang   Group: iGEM21_HK_SSC   (2021-10-01)
Revision as of 14:31, 17 October 2021 by Meowstarperson (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


panS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1131
    Illegal AgeI site found at 2856
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

repB and repA in this part have been codon optimized for Synechococcus elongatus UTEX 2973.

Source

It comes from the pANS plasmid in Synechococcus elongatus PCC7942.(Chen et al., 2016)

References

Chen, Y., Taton, A., Go, M., London, R. E., Pieper, L. M., Golden, S. S., & Golden, J. W. (2016). Self-replicating shuttle vectors based on pANS, a small endogenous plasmid of the unicellular cyanobacterium Synechococcus elongatus PCC 7942. Microbiology, 162(12), 2029–2041. https://doi.org/10.1099/mic.0.000377