Primer

Part:BBa_K3817008:Design

Designed by: Sun Ji   Group: iGEM21_UCAS-China   (2021-10-01)
Revision as of 15:36, 1 October 2021 by SunJi UCAS (Talk | contribs) (→‎Design Notes)

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Pbluescript Ⅱ SK+ MCS forward primer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We purposely designed the primers by NCBI and our method in order for them to bind specifically, and finally ensured that the difference in annealing temperature Tm between the forward and reverse primers was extremely small.

Source

We design this primer.

References