![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
KS final
Part:BBa_K4060117:Design
Designed by: YU CHUAN LEE Group: iGEM21_NYCU-Taipei (2021-10-01)
2021 NYCU Taipei final kill switch design
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 245
Illegal EcoRI site found at 873
Illegal XbaI site found at 261
Illegal XbaI site found at 889
Illegal SpeI site found at 515
Illegal SpeI site found at 1232 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 245
Illegal EcoRI site found at 873
Illegal NheI site found at 125
Illegal NheI site found at 145
Illegal NheI site found at 168
Illegal NheI site found at 793
Illegal NheI site found at 813
Illegal NheI site found at 836
Illegal SpeI site found at 515
Illegal SpeI site found at 1232
Illegal NotI site found at 252
Illegal NotI site found at 880 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 245
Illegal EcoRI site found at 873
Illegal BamHI site found at 65
Illegal BamHI site found at 312
Illegal BamHI site found at 733 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 245
Illegal EcoRI site found at 873
Illegal XbaI site found at 261
Illegal XbaI site found at 889
Illegal SpeI site found at 515
Illegal SpeI site found at 1232 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 245
Illegal EcoRI site found at 873
Illegal XbaI site found at 261
Illegal XbaI site found at 889
Illegal SpeI site found at 515
Illegal SpeI site found at 1232 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The construction of this part is according to igem assembly protocol.
Source
The MazE and MAzF gene are cloned from E.coli MG1655. The remaining parts are from 2019 igem kits.