Composite
KS final

Part:BBa_K4060117:Design

Designed by: YU CHUAN LEE   Group: iGEM21_NYCU-Taipei   (2021-10-01)
Revision as of 13:30, 7 December 2021 by JasonCTS (Talk | contribs)

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2021 NYCU Taipei final kill switch design


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 245
    Illegal EcoRI site found at 873
    Illegal XbaI site found at 261
    Illegal XbaI site found at 889
    Illegal SpeI site found at 515
    Illegal SpeI site found at 1232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 245
    Illegal EcoRI site found at 873
    Illegal NheI site found at 125
    Illegal NheI site found at 145
    Illegal NheI site found at 168
    Illegal NheI site found at 793
    Illegal NheI site found at 813
    Illegal NheI site found at 836
    Illegal SpeI site found at 515
    Illegal SpeI site found at 1232
    Illegal NotI site found at 252
    Illegal NotI site found at 880
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 245
    Illegal EcoRI site found at 873
    Illegal BamHI site found at 65
    Illegal BamHI site found at 312
    Illegal BamHI site found at 733
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 245
    Illegal EcoRI site found at 873
    Illegal XbaI site found at 261
    Illegal XbaI site found at 889
    Illegal SpeI site found at 515
    Illegal SpeI site found at 1232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 245
    Illegal EcoRI site found at 873
    Illegal XbaI site found at 261
    Illegal XbaI site found at 889
    Illegal SpeI site found at 515
    Illegal SpeI site found at 1232
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction of this part is according to igem assembly protocol.


Source

The MazE and MAzF gene are cloned from E.coli MG1655. The remaining parts are from 2019 igem kits.

References