Coding
Part:BBa_K3989001:Design
Designed by: Song-Yuan Zhang Group: iGEM21_UZurich (2021-09-30)
uTEV3, efficiency-enhanced TEV protease variant
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sanchez, M. I., & Ting, A. Y. (2020). Directed evolution improves the catalytic efficiency of TEV protease. Nature methods, 17(2), 167-174.
Source
Original TEV protease sequence and structure. PDB: 1LVM
References
1) Denard, C. A., Paresi, C., Yaghi, R., McGinnis, N., Bennett, Z., Yi, L., ... & Iverson, B. L. (2021). YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants. ACS Synthetic Biology, 10(1), 63-71.