Coding

Part:BBa_K3739083

Designed by: Shichen Geng   Group: iGEM21_XMU-China   (2021-09-30)
Revision as of 18:57, 30 September 2021 by Gengshichen (Talk | contribs)


LMT-his-hutH-FT

Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. LMT is a signal peptile. Direct fluorescent labeling of tetracysteine-motif-tagged secretory proteins with the biarsenical compound FlAsH is possible in situ without the need to recover the cell-free supernatant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 219
    Illegal NgoMIV site found at 655
    Illegal NgoMIV site found at 1390
    Illegal NgoMIV site found at 1626
    Illegal AgeI site found at 292
    Illegal AgeI site found at 1119
    Illegal AgeI site found at 1615
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None