Composite

Part:BBa_K3739084

Designed by: Shichen Geng   Group: iGEM21_XMU-China   (2021-09-30)
Revision as of 17:19, 20 October 2021 by Gengshichen (Talk | contribs)


K525998(T7-RBS)-Aly01-his-hutH-FT

Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. Aly01 is a signal peptile. The proteins labeled of tetracysteine motif tag (FlAsH tag, FT in short) can be detected with the biarsenical compound FlAsH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 275
    Illegal NgoMIV site found at 711
    Illegal NgoMIV site found at 1446
    Illegal NgoMIV site found at 1682
    Illegal AgeI site found at 111
    Illegal AgeI site found at 348
    Illegal AgeI site found at 1175
    Illegal AgeI site found at 1671
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None