Composite
Pre B2
Part:BBa_K4060105:Experience
Designed by: YU CHUAN LEE Group: iGEM21_NYCU-Taipei (2021-09-30)
Revision as of 14:24, 7 December 2021 by Clairechen (Talk | contribs)
Assembly of PreB2 (pSB1C3)
The gene fragment Pre B2 is composed of Pre A2 and the Thermometer RBS BBa_K115002. We digested the vector fragment BBa_K115002 with EcoRI and XbaI and the insert fragment Pre A2 with EcoRI and SpeI. We ligate these fragments together to form Pre B2.
Fig.6 Agarose electrophoresis in order to verify Pre B2. Lane 1, 100bp ladder.Lane 1’, partial of Life 1 kb Plus ladder. Lane 2,3, Pre B2 digested with NotI.
Applications of BBa_K4060105
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