Coding
NphT7

Part:BBa_K3726000

Designed by: Jorge Fernández Méndez   Group: iGEM21_MADRID_UCM   (2021-08-03)
Revision as of 09:58, 2 October 2021 by Fmjorge99 (Talk | contribs)

(diff) ↠Older revision | Latest revision (diff) | Newer revision → (diff)

CDS_NphT7

This part corresponds with the protein coding sequence for an Acetoacetyl CoA synthase.

The codified enzyme from Streptomyces sp. CL190.(NphT7) catalyzes the irreversible condensation-decarboxylation step of 1 molecule of acetyl-coA with Malonyl-coA to transform them into Acetoacetyl-coA and CO2.

Sequence & Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None