Composite

Part:BBa_K3745040

Designed by: Jianfei Song   Group: iGEM21_BNDS_China   (2021-09-30)
Revision as of 14:53, 11 October 2021 by Jianfei Song (Talk | contribs)


RhlABC + tags + scaffold

This plasmid contains rhlA,B,C as well as a tag and scaffold to connect these three enzymes together, and it is the most essential plasmid in our protein scaffold project. By connecting rhlA,B,C together, we successfully improved the reaction efficiency of the enzymes that assemble rhamnolipid by maximizing the collision probability of substrates and enzymes as well as minimizing the time interview between each reaction, hence increase rhamnolipid yield.

For the detailed function of each enzyme, see BBa_K3745030


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2281
    Illegal BamHI site found at 6640
    Illegal XhoI site found at 6649
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2149
    Illegal NgoMIV site found at 2554
    Illegal NgoMIV site found at 4123
    Illegal NgoMIV site found at 5446
    Illegal NgoMIV site found at 5761
    Illegal NgoMIV site found at 6076
    Illegal NgoMIV site found at 6391
    Illegal AgeI site found at 3166
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5676
    Illegal BsaI site found at 5991
    Illegal BsaI site found at 6306
    Illegal BsaI site found at 6621
    Illegal SapI.rc site found at 4262
    Illegal SapI.rc site found at 5305


[edit]
Categories
Parameters
None