Part:BBa_K3822005

miR196a-148a 2.0

miR196a-148a 2.0 is an improvement of Part BBa_K3160007. which could monitor the expression of miRNAs in gastric cancer cells

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

1.Construction of the plasmids

The miRNA “ sponge” method was introduced by several years ago as a means to create continuous miRNA loss of function in cell lines and transgenic organisms. More effective are sponges containing bulged sites that are mispaired opposite miRNA positions 9-12 (Ebert et al. 2007, Gentner et al. 2009), presumably because they form a more stable interaction with the miRNA. pEGFP-miR196a-148a 2.0(Part K3822005) was designed based on the principle of effective sponges containing bulged sites (Fig. 1). We compare pEGFP-miR196a-148a 2.0 with pEGFP-miR196a-148a 1.0 (Part K3160007,designed by a 2019 iGEM team) on monitoring the expression of miRNAs in gastric cancer cells.