Coding

Part:BBa_K3805138:Design

Designed by: Yumo Li   Group: iGEM21_BNU-China   (2021-09-29)
Revision as of 13:24, 18 October 2021 by MUSong (Talk | contribs)


agrBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chose to amplify the desired fragment from the Staphylococcus aureus genome, and since the agrB and agrD are very close together in this strain, we amplified this fragment according to a single element.

According to our pathway design (below), specific binding of AIP to a protein on the guard cell membrane activates the activity of this membrane protein, AgrC, which drives AgrD phosphorylation and activates the P2 promoter, thereby driving the release of mcherry to the extracellular compartment. Therefore, if you want to test the concentration of AIP, you need to measure the amount of mcherry

The experimental design was divided into two steps. Step 1: Obtaining standard data for AIP Add a specified amount of AIP solution to the guard's bacterial solution and measure the amount of mcherry Step 2: Determine the amount of mcherry in the deceiver and fit the actual process to the deceiver AIP yield based on the standard data


Source

This part contains the coding regions for group I agrB and agrD (in that order) from S. aureus strain NCTC4137.

References