Coding

Part:BBa_K3787003

Designed by: Leung Gabriel, Tam Ching Yeung, Wong Chun Hei   Group: iGEM21_HK_GTC   (2021-09-29)
Revision as of 06:47, 6 October 2021 by Ian Tam GTEYC HK (Talk | contribs)


I.s. MHETase

MHETase from Ideonella Sakaiensis. The sequence is optimized for the expression of Escherichia coli, strains DH5[alpha] and C41(DE3)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 983
    Illegal PstI site found at 1012
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 132
    Illegal PstI site found at 983
    Illegal PstI site found at 1012
    Illegal NotI site found at 680
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 983
    Illegal PstI site found at 1012
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 983
    Illegal PstI site found at 1012
    Illegal NgoMIV site found at 607
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None