Coding

Part:BBa_K3733006:Design

Designed by: Zhenhao Han   Group: iGEM21_HZAU-China   (2021-09-28)
Revision as of 12:14, 28 September 2021 by HanZhenhao (Talk | contribs) (→‎References)

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ScGS: Streptomyces coelicolor geosmin synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization has been applied on the 2181 bp sequence from Streptomyces coelicolor A3(2) to improve gene expression.


Source

The original sequence SCO6073 comes from Genbank(NC_003888).

References

Harris G G, Lombardi P M, Pemberton T A, et al. Structural Studies of Geosmin Synthase, a Bifunctional Sesquiterpene Synthase with αα Domain Architecture That Catalyzes a Unique Cyclization–Fragmentation Reaction Sequence[J]. Biochemistry, 2015, 54(48): 7142-7155.