Regulatory

Part:BBa_K3788017:Experience

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-28)
Revision as of 14:32, 21 October 2021 by Rpages (Talk | contribs)


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Applications of BBa_K3788017

Methods

In order to characterize the promoter strength, we fused it to the GFP encoding sequence BBa_E0040 and to the timer-lysis device part BBa_K3788018 (composite part BBa_K3788019). Induction of the SOS response was induced by mitomycin C treatments and the fluorescence was recorded in a dose-dependent manner using a microplate reader. Following iGEM Measurement recommendations, the GFP fluorescence intensity was converted to a concentration of fluorescein protein thanks to a standard curve of fluorescein dilution series.


Results

The cells carrying the BBa_K3788019 construct were grown with various concentrations of mitomycin, ranging from 0 to 800 ng.ml-1. The OD (600 nm) and the amount of green fluorescence were recorded over time.




User Reviews

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