Coding

Part:BBa_K3788004

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)
Revision as of 15:42, 15 October 2021 by Rpages (Talk | contribs)


6His-P20, Flag-Cry11Aa and Strep-Cyt1Aa coding sequence

The Part BBa_K3788004 is a part composed with BBa_K3788003 (6His_P20__Flag_cry11Aa) and BBa_K3788000 (Strep_cyt1Aa). Theses 3 proteins proteins are Bacillus thuringiensis proteins. P20 chaperon have a histidin tag in Nter, Cry11Aa toxin have a Flag tag in Nter and Cyt1Aa toxin have a Strep tag II in Nter to allow to purified proteins P20, Cyt1Aa and Cry11Aa; and allow the detection and specific identification by WesterBlot.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 154
    Illegal XhoI site found at 2438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None