Coding

Part:BBa_K3788003:Experience

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)
Revision as of 15:20, 15 October 2021 by Rpages (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3788003

PCR amplification of 6hisp20_flagCry11Aa genes:

The DNA sequence of flagcry11Aa and 6hisp20 were amplified by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is 2540bp and a 2000bp band is observed . Amplification of DNAs sequences with primers were validated.

T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png

Figure. Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning. a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 pB), flagcry11Aa (expected 1847 pB), strepcyt1Aa (expected 775 pB) and 6hisp20 (expected 568 pB); L (SMART Ladder, its size is shown on the right side).



User Reviews

UNIQ5d074fba7e10e630-partinfo-00000000-QINU UNIQ5d074fba7e10e630-partinfo-00000001-QINU