Part:BBa_K4040024

pTET-mCherry-PGK-CD20 Expression Cassette

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1649
  Illegal PstI site found at 2560
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 1649
  Illegal PstI site found at 2560
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 1649
  Illegal PstI site found at 2560
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1649
  Illegal PstI site found at 2560
  Illegal NgoMIV site found at 2407
  Illegal AgeI site found at 1115
  Illegal AgeI site found at 1227
  Illegal AgeI site found at 1514
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2654
  Illegal BsaI.rc site found at 2723
  Illegal BsaI.rc site found at 2729
  

Usage and Biology

The composite part consists of seven parts: the promoter pTET(BBa_K1061013) which initiates the expression of coding sequence. Fluorescent marker mCherry (BBa_J04450) which detects the expression of the pathway and the expression component of CAR-MERTK: CD8a signal peptide (BBa_K4040014), CR3022 scFv (BBa_K4040019), CD8 Hinge (BBa_K4040004), CD8TM (BBa_K4040007), MERTK(BBa_K4040002).  When the tTA fragment from IL-6 complex receptor BBa_K4040022 is released, it binds to the promoter TET to promote the expression of CAR-MERTK and facilitate the transformation of macrophages into M2 state to alleviate the inflammatory response.

Figure 1.The coding of the CAR-MERTK.

Figure 2.The structure and downstream pathway of the composite IL6R.