Part:BBa_K3832001

Induced expression circuit of aroG (Mutant s211f)

This Part is the Induced expression circuit of CDS aroG (Mutant s211f) (BBa_K3832000).It contains lacUV5 promoter and a whole expression unit of lacI protein, thus aroG-s211f can be induced by IPTG.

Our team use GoldenGate assembly to assemble the subparts into this circuit.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2554
  Illegal NheI site found at 2577
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2541
  Illegal XhoI site found at 1146
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 453