Coding

Part:BBa_K3832000:Design

Designed by: Hongyu Chen,Dan Tan   Group: iGEM21_XJTU-China   (2021-09-26)
Revision as of 04:14, 14 October 2021 by My Elliot (Talk | contribs)

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aroG (Mutant S211F)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 934
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The function of the mutant should be same as the wild-type, while the efficiency can not be reduced. The mutated site is required to remove the allosteric effect of Phe without affecting the catalytic activity of the enzyme


Source

AroG (Mutant S211F) is derived from a wild-type point mutation. CDS of Wild-type aroG can be gained from genomic sequence of E.coli DH5alpha strain.


References